Published Online 17 February 2011
Science 4 March 2011:
Vol. 331 no. 6021 pp. 1159-1165
DOI: 10.1126/science.1202393
RESEARCH ARTICLE
Crystal Structure of the Dynein Motor Domain
Andrew P. Carter1,2,*†, Carol Cho1,*, Lan Jin1, and Ronald D. Vale1,†
+Author Affiliations
1Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California–San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
2Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK.
†To whom correspondence should be addressed.
E-mail: cartera@mrc-lmb.cam.ac.uk (A.P.C.); vale@cmp.ucsf.edu(R.D.V.)
↵* These authors contributed equally to this work.
ABSTRACT
Dyneins are microtubule-based motor proteins that power ciliary beating, transport intracellular cargos, and help to construct the mitotic spindle. Evolved [???] from ring-shaped hexameric AAA-family adenosine triphosphatases (ATPases), dynein’s large size and complexity have posed challenges for understanding its structure and mechanism. Here, we present a 6 angstrom crystal structure of a functional dimer of two ~300-kilodalton motor domains of yeast cytoplasmic dynein. The structure reveals an unusual asymmetric arrangement of ATPase domains in the ring-shaped motor domain, the manner in which the mechanical element interacts with the ATPase ring, and an unexpected interaction between two coiled coils that create a base for the microtubule binding domain. The arrangement of these elements provides clues as to how adenosine triphosphate–driven conformational changes might be transmitted across the motor domain.
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NOTA DESTE BLOGGER:
Os autores fizeram a ousada afirmação que as dineínas evoluíram das "ring-shaped hexameric AAA-family adenosine triphosphatases (ATPases)", mas não nos disseram COMO que isso ocorreu na história evolucionária das dineínas.
Fui, nem sei por que, pensando como é fácil fazer ciência normal sob a orientação de uma teoria científica, a Síntese Evolutiva Moderna, considerada morta desde 1980. Basta escrever evoluiu... Simples, simples, simples!!!
