The genome of the tardigrade Hypsibius dujardini
Georgios Koutsovoulos, Sujai Kumar, Dominik R Laetsch, Lewis Stevens, Jennifer Daub, Claire Conlon, Habib Maroon, Fran Thomas, Aziz Aboobaker, Mark Blaxter
doi: http://dx.doi.org/10.1101/033464
Abstract
Tardigrades are meiofaunal ecdysozoans and are key to understanding the origins of Arthropoda. We present the genome of the tardigrade Hypsibius dujardini, assembled from Illumina paired and mate-pair data. While the raw data indicated extensive contamination with bacteria, presumably from the gut or surface of the animals, careful cleaning generated a clean tardigrade dataset for assembly. We also generated an expressed sequence tag dataset, a Sanger genome survey dataset and used these and Illumina RNA-Seq data for assembly validation and gene prediction. The genome assembly is ~130 Mb in span, has an N50 length of over 50 kb, and an N90 length of 6 kb. We predict 23,031 protein-coding genes in the genome, which is available in a dedicated genome browser at http://www.tardigrades.org. We compare our assembly to a recently published one for the same species and do not find support for massive horizontal gene transfer. Additional analyses of the genome are ongoing.
Copyright
The copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license.
FREE PDF GRATIS: bioRxiv
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Vide pesquisa publicada antes no PNAS, afirmando a extensa transferência horizontal de gene:
Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade
Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade
Thomas C. Boothby et al.
Quem está certo? Boothby et al ou Koutsovoulos et al???
