Cell Reports
Volume 3, Issue 4, p1105–1116, 25 April 2013
A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules
Andres J. Lopez-Contreras 7, Isabel Ruppen 7, Maria Nieto-Soler, Matilde Murga, Sara Rodriguez-Acebes, Silvia Remeseiro, Sara Rodrigo-Perez, Ana M. Rojas, Juan Mendez, Javier Muñoz, Oscar Fernandez-Capetillo correspondence email
7These authors contributed equally to this work and are co-first authors
Open Access
Publication History
Published Online: March 28, 2013 Accepted: March 8, 2013
Received in revised form: February 13, 2013 Received: November 28, 2012
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Highlights
- A comprehensive resource of replisome and replisome-associated factors
- A putative link between Williams-Beuren syndrome and DNA replication
- SUMO and UQ show opposite concentrations around replisomes
- ZNF24 is a newly identified DNA-replication factor
Summary
DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.
Received: November 28, 2012; Received in revised form: February 13, 2013; Accepted: March 8, 2013; Published Online: March 28, 2013
© 2013 The Authors. Published by Elsevier Inc.
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